HPLC法测定双花颗粒中绿原酸的含量

Content Determination of Chlorogenic Acid in Shuanghua Granules by HPLC

目的:建立测定双花颗粒中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Shimpack VP-ODS(150mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸(10:90),检测波长为326nm,流速为1.0m L.min-1,柱温为30℃。结果:绿原酸的进样浓度在5.4～54.0μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9997);平均加样回收率为99.05%,RSD=4.22%(n=9)。结论:本方法准确、简便、可靠,可用于双花颗粒的质量控制。

OBJECTIVE：To establish the method for the content determination of chlorogenic acid in Shuanghua granules.METHODS：HPLC method was adopted.Chromatographic conditions were as follows：Shimpack VP-ODS（150 mm×4.6 mm,5 μm）column was used with mobile phase consisted of acetonitrile-0.2% phosphoric acid（10：90） at a flow rate of 1.0 mL·min-1 under a detection wavelength at 326 nm.The column temperature was 30 ℃.RESULTS：The linear range of chlorogenic acid was 5.4～54.0 μg·mL-1（r=0.999 7） with an average recovery rate of 99.05%（RSD=4.22%,n=9）.CONCLUSION：The method is simple,reliable and accurate,and can be used for the quality control of Shuanghua granules.