RP-HPLC法测定不同采收期广东紫珠、大叶紫珠中木犀草素的含量

Content Determination of Luteolin in Callicarpa kwangtungensis and Callicarpa macrophylla in Different Harvesting Time by RP-HPLC

目的:测定不同采收期广东紫珠、大叶紫珠中木犀草素的含量并探讨木犀草素含量的动态变化规律。方法:采用反相高效液相色谱法。色谱柱为Alltech C18(250 mm×4.6 mm,5μm),柱温为30℃,流动相为甲醇-0.3%磷酸水(47∶53),检测波长为352nm,流速为1 mL·min-1。结果:木犀草素进样量在0.015～0.300μg范围内与峰面积积分值呈良好的线性关系(r=0.999 9);平均加样回收率为98.52%,RSD=1.84%(n=6)。随着生育期的延长,木犀草素含量逐渐增高,生长末期含量达到最高;大叶紫珠中木犀草素的含量普遍高于广东紫珠。结论:本试验结果可为广东紫珠、大叶紫珠2种药材的半野生人工抚育及人工栽培提供科学依据。

OBJECTIVE：To determine the content of luteolin in Callicarpa kwangtungensis and Callicarpa macrophylla in different harvesting time and to explore the rules of dynamic change of luteolin content.METHODS：RP-HPLC was performed on Alltech C18（250 mm×4.6 mm,5 μm） column with column temperature at 30 ℃.The mobile phase consisted of methanol-0.3% phosphate aqueous solution（47：53） at flow rate of 1 mL·min-1,and the detection wavelength was set at 352 nm.RESULTS：The linear range of luteolin was 0.015～0.300 μg（r=0.999 9） with an average recovery of 98.52%（RSD=1.84%,n=6）.The content of luteolin increased gradually with the growth of plants and reached the peak at the end of growth period.The content of luteolin in C.macrophylla was commonly higher than in C.kuangtungensi.CONCLUSION：Results of study can provide scientific basis for natural cultivation or artificial cultivation of C.kwangtungensi and C.macrophylla.