HPLC法测定活血散瘀灌肠液中丹参素的含量

Content Determination of Danshensu in Huoxue Sanyu Enema by HPLC

目的:建立测定活血散瘀灌肠液中丹参素含量的方法。方法:采用高效液相色谱法。色谱柱为Extend-C18(250 mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(7∶92.5∶0.5),流速为1.0 mL·min-1,检测波长为280 nm。结果:丹参素检测浓度在0.01～0.10mg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.999 9);平均回收率为99.88%,RSD=1.08%(n=6)。结论:本方法简便、快速、准确,可用于活血散瘀灌肠液中丹参素的含量测定以及质量控制。

OBJECTIVE：To establish the method for the content determination of tanshinol in Huoxue sanyu enema.METHODS：HPLC method was adopted.Extend-C18（250 mm×4.6 mm,5 μm） column was used with mobile phase consisted of methanol-water-glacial acetic acid（7：92.5：0.5） at flow rate of 1.0 mL·min-1.The detection wavelength was set at 280 nm.RESULTS：The linear range of danshensu was 0.01～0.10 mg·mL-1（r=0.999 9） with an average recovery of 99.88%（RSD=1.08%,n=6）.CONCLUSION：The method is simple,rapid and accurate for the content determination and quality control of danshensu in Huoxue sanyu enema.