核桃楸树皮乙醇提取物乙酸乙酯相的质谱分析

Mass Spectrum Analysis of Acetic Ether Part of Ethanol Extracts of Juglans mandshurica Stem-barks

目的:为进一步开发和利用核桃楸树皮的药用成分提供参考。方法:从核桃楸树皮70%的乙醇回流提取物中,分别经溶剂分级萃取,取乙酸乙酯萃取相,经硅胶柱色谱分离,得到2个条带,收集后进行质谱分析。结果:供试品Ⅰ的2个主要成分的分子量在317和437左右,供试品Ⅱ的3主要成分分子量在303、437和453左右,其中质谱分子量303、317、437的组分在供试品Ⅰ和Ⅱ中均有出现。结论:初步判定两样品中可能含有槲皮素及杨梅苷,另外还可能含新的未知成分,需要做进一步的分离鉴定。

OBJECTIVE：To provide reference for the further development and application of medicinal constituents of Juglans mandshurica stem-barks.METHODS：70% ethanol extracts of J.mandshurica stem-barks were extracted by solvent classification extraction and ethyl acetate extraction phase was separated on silica gel column.Two compositions were isolated and these compositions were analyzed by mass spectrum.RESULTS：The molecular weight of two main compositions in sample I were about 317 and 437,and that of three main compositions of sample Ⅱ were about 303,437 and 453,respectively.The molecular weight of these components 303,317 and 437 had appeared in the sample I and Ⅱ.CONCLUSION：Two samples maybe contain quercetin and myricetin in consideration of a preliminary analysis.New constituents maybe discovered,which require further separation and identification.