摘要
目的探讨多索茶碱对支气管哮喘（简称哮喘）大鼠肺部炎症的影响，以及在哮喘发病时基质金属蛋白酶9在肺部沉积的影响。方法①免疫组织化学法检测基质金属蛋白酶9在肺部沉积的情况；②肺组织学检查；③酶联免疫吸附实验检测外周血总IgE水平。结果哮喘组支气管肺泡灌洗液中及血清巾IgE的水平明显高于对照组（P〈0．05）；基质金属蛋白酶－9在肺部的沉积也明显多于对照组；而多索茶碱干预组IgE水平以及外周血总IgE水平明显低于哮喘组（P〈o．05），肺组织病变减轻，基质金属蛋白酶9的沉积也明显减少（P〈o．05）。结论多索茶碱能减少基质金属蛋白酶－9在肺部的沉积，延缓气道重塑的发展。
Objective To investigate effect of theophylline on inflammatory airway of asthmatic mouse, and depositive effect of metalloprotease -9 when patient fall in asthma. Methods ①Detect matrix metalloproteinase 9 deposit in bellows （immunohistochemical method ）. ② Inspection of bellows histological anatomy.③Detect the contents IgE of peripheral blood （ELISA）. Results The group of asthma＇s level of IgE in bronchoalveolar lavage fluid higher than control group （ P 〈0.05）. The deposit of matrix metalloproteinase-9 in asthma more than control group. However,the contents of level of IgE in group of theophylline intervention were lower than in asthma （P〈0.05）. The deposit of matrix metalloproteinase 9 have decrease obviously （P 〈7 0.05 ）. Conclusions Theophylline can lessen the deposit of matrix metalloproteinase-9 have decrease obviously （ P 〈0.05）.
出处
《国际呼吸杂志》
2011年第11期806-809,共4页
International Journal of Respiration
