一株海洋细菌对中肋骨条藻的溶解效应及其溶藻特性

Lysis effect and mechanisum of one bacterial strain on Skeletonema costatum

从山东胶州湾分离得到1株海洋溶藻菌,暂将其命名为JZ-1.根据生理生化及16S rDNA序列分析鉴定,菌株JZ-1属于交替单胞菌属(Alteromonas).研究表明,菌株JZ-1对中肋骨条藻具有很好的溶解效果,它能够破坏藻细胞膜内物质的结构和细胞膜的完整性,使细胞膜内物质流出导致藻细胞死亡.溶藻现象发生在细菌培养液的上清液和0.2μm的过滤液中,而不是在细菌菌体中,这表明菌株JZ-1通过分泌代谢物对中肋骨条藻产生溶解作用,且当菌株JZ-1由对数生长期向稳定期过渡时,其代谢物的溶藻率达到最大.代谢物分子量<5kD,具有热稳定性、耐酸性,但不耐碱.

A strain of marine algae-lysing bacterium designated as JZ-1 was isolated from Jiaozhou Bay,China.Strain JZ-1 showed highly homology with Alteromonas sp.on the basis of morphological and biochemical characteristics and16S rDNA sequence analysis.Strain JZ-1 showed significant algicidal activity against Skeletonema costatum,it broke the materials structures in membrane and membrane integrity,and the materials leak through membrane to make the cells die.In addition,algicidal activity against S.costatum was detected in the filtrate and after filter through 0.2 μm syringe filter,not in the cells.Strain JZ-1 effects S.costatum by releasing certain algicidal substances,and this point was also confirmed by paper disk method.The metabolite had the highest algicidal activity at this moment when the bacterium transited to stationary phase from logarithmic growth phase.We tested some characteristics of the metabolite and the results were as follows： the molecular weight of metabolite was5kD;the metabolite had certain acid tolerance but no alkali tolerance;the metabolite had thermal stability.