摘要
目的 研究实验性狗溃疡病手术后G细胞密度和功能的变化。方法 给成年杂种狗分别施行胃大部切除、胃空肠吻合术(DPG-BⅡ)或保留幽门括约肌胃段切除术(PPSG)或高选择性迷走神经切断术(HSV)。以中和滴定法测定胃酸;以放射免疫法测定血清胃泌素;用免疫组化方法检测G细胞数量和功能。结果 三种手术均能有效抑制胃酸分泌。DPG-BⅡ和PPSG术后血清胃泌素降低,而HSV术后血清胃泌素升高。三种手术后胃窦或残留胃窦、十二指肠和空肠的G细胞数密度增加、功能增强。结论 溃疡病术后G细胞呈代偿性增生和功能增强,胃泌素水平不随胃酸降低而相应降低。
Objective To study effects of operations of peptic ulcer for dogs on G-cell density and function. Methods Hibrid dogs had undergone distal partial gastrectomy with Billroth II anastomosis (DPG-BII), pylorus-preserving segmental gastrectomy (PPSG) or highly selective vagotomy. Gastric acid and serum gastrin were measured with neutralization method and radioimmunoassay. respectively. G-cell density and function were determined by immunohistochemical method. Results Gastric acid output was effectively inhibited by all above three operations. Subsequent to DPG-BII and PPSG serum gastrin, which increased after HSV, decreased. Postoperatively the density of G-cell, whose function enhanced, increased in antrum, duodenum and jejunum. Conclusion Complementary G-cell hyperplasia and hyperfunction are present after operations of peptic ulcer, serum gastrin do not changed in pace with the decrease of gastric acid.
