丝素蛋白/硅酸钙复合纳米纤维的结构与性能

Structures and properties of silk fibroin/CaSiO_3 composite nanofibers

采用静电纺丝法制备了丝素蛋白/硅酸钙复合纳米纤维。XRD和FT-IR分析表明,复合纤维材料中丝素的结构以SilkⅡ为主。复合纤维的直径为200～400 nm,孔隙率约为78%。水接触角实验结果显示,复合纤维的亲水性比纯丝素纤维有所提高。分别用模拟体液和Tris缓冲溶液浸泡实验研究了复合纳米纤维的体外生物活性和降解性,结果表明,质量分数为20%的硅酸钙/丝素蛋白复合纳米纤维在模拟体液中浸泡7 d后,表面覆盖了较厚的碳酸羟基磷灰石层,其在Tris缓冲溶液中浸泡12周后的降解率达42.4%,具有良好的生物活性和降解性。研究结果显示,丝素蛋白/硅酸钙复合纳米纤维有望作为生物活性骨组织修复材料。

The silk fibroin（SF）/CaSiO3（CS） composite nanofiber was fabricated via electrospinning.XRD and FT-IR analysis showed that Silk II governed the structure of the SF/CS composite nanofiber.It has a diameter of 200-400 nm and porosity of about 78%.Water contact angle experiment indicated that it has better hydrophilicity than the pure SF nanofiber.The in vitro bioactivities and degradability of SF/CS nanofiber were investigated using experiments of soaking in simulated body fluid（SBF） and respectively Tris-buffer.After being soaked in SBF for 7 days,the surfaces of the composite nanofibers with 20% CaSiO3 were covered with a hydrocarbonate apatite layer.The degradability of this sample reached 42.4% after being soaked in Tris-buffer for 12 weeks,showing its good bioactivity and degradability.This composite SF/CS nanofiber provides a promising opportunity for use as bioactive bone tissue repair material.