一氧化氮对肺泡巨噬细胞致炎效应的调节作用

Effect of nitric oxide on inflammatory response of alveolar macrophages

目的 探讨一氧化氮（ＮＯ）对肺泡巨噬细胞致炎效应的调节作用。方法 内毒素（ＬＰＳ）刺激体外培养的小鼠肺泡巨噬细胞，观察ＴＮＦα等细胞因子释放及ＮＯ对细胞因子释放的影响。结果 ＬＰＳ诱导肺泡巨噬细胞释放炎症性细胞因子的时效关系不同，肺泡巨噬细胞释放ＴＮＦα、ＩＬ－１β、ＩＬ－６的峰值时间分别为６、８、２４ 小时。ＮＯ合成酶抑制剂甲基异硫脲明显降低ＬＰＳ诱导的ＮＯ释放，同时强烈刺激肺泡巨噬细胞释放ＩＬ－１β、ＩＬ－６，但对ＴＮＦα释放无明显影响。外源性补充ＮＯ明显抑制ＩＬ－Ｉβ、ＩＬ－６ 释放，亦明显抑制ＩＬ－１０ 释放。结论 内源性及外源性ＮＯ对肺泡巨噬细胞炎症性细胞因子释放具有明显抑制作用。

Objective To observe the regulatory effects of nitric oxide (NO) on inflammatory reaction of alveolar macrophage.Methods Alveolar macrophages from mice were stimulated with LPS 10mg·L -1 and S methylisothiorea sulfate(SMT) or S nitroso N acetyl D,L pennicillamine (SNAP).Results Following LPS stimulation,TNFα,IL 1 β and IL 6 levels peaked at the 6th h,12th h and 24th h respectively in alveolar macrophages.SMT inhibited LPS induced NO release and resulted in an increase of IL 1β and IL 6 secretion in alveolar macrophages,whereas the TNFα levels remained unchanged.SNAP inhibited IL 1β and IL 6 secretion in a dose dependent manner,but exerted no effect on TNFα release.Conclusion Endogenous and exogenous NO can inhibit the LPS induced inflammatory cytokines secretion.