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动态高压微射流对猪胰脂肪酶的影响

Effect of dynamic high-pressure microfluidization on porcine pancreatic lipase
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摘要 以猪胰脂肪酶(PPL)为原料,研究动态高压微射流(DHPM)对酶活性和构象的影响。结果显示:DHPM处理对PPL具有钝化作用,处理压力越大,钝化作用越小;4℃下贮存24h,相对酶活有所回升。通过紫外光谱和圆二色谱分析DHPM对PPL构象的影响,发现经100MPa DHPM处理后,PPL的紫外吸收强度降低,β-折叠含量减少,但随着处理压力增大,紫外吸收强度和β-折叠含量逐渐增大;4℃放置24h后,紫外吸收强度和β-折叠含量进一步上升。PPL的相对酶活与紫外吸收强度、β-折叠含量呈一定的正相关。 The effect was studied of dynamic high-pressure microfluidization（DHPM） treatment on porcine pancreas lipase（PPL） enzyme activity and conformation.PPL was inactivated after DHPM treatment and the higher pressure,the less inactivationis.The relative activity increased during 24 h storage at 4 ℃.UV and CD spectra analysis showed,after DHPM treatment in 100 MPa,the UV absorption and β-sheet content reduced.Yet the UV absorption and β-sheet content increased with the increase of treat pressure.After 24 h storage at 4 ℃,the UV absorption and β-sheet content was further increased.The results showed that the relative activity was correlated with UV absorption and β-sheet content positively.
作者 尹曼 刘伟 钟业俊 刘成梅 黄波
机构地区 南昌大学食品科学与技术国家重点实验室 南昌大学生物质转化教育部工程研究中心
出处 《食品与机械》 CSCD 北大核心 2011年第3期3-6,共4页 Food and Machinery
基金 国家自然科学基金资助项目(编号:31000209) 食品科学与技术国家重点实验室目标导向项目(编号:SKLF-MB-201004)
关键词 动态高压微射流技术 猪胰脂肪酶 酶活 构象 dynamic high-pressure microfluidization porcine pancreatic lipase activity conformation
分类号 TS201.25 [轻工技术与工程—食品科学]
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参考文献16

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食品与机械
2011年 第3期

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