HPLC测定荷叶中碱盐的含量

Study on determination of nuciferine salt by HPLC

以荷叶为原料,建立一种荷叶碱盐含量的测定方法。运用高效液相色谱法测定,色谱柱为Hypersil ODS2(4.6mm×250mm,5μm),流动相为乙腈-磷酸盐缓冲溶液(0.05%磷酸-0.2%三乙胺-水)(25∶75,V/V),流速为1.0mL/min,检测波长为270nm。结果显示,荷叶碱盐在0.053～0.159mg/mL(R2=0.999 2)范围内具有良好的线性关系,平均回收率为96.4%,RSD为0.05%。该方法简便、准确,灵敏度高,重复性好,可用于荷叶碱盐成分的含量测定。

To establish an HPLC method for the determination of nuciferine salt on lotus leaf.Methods： Analyses were carried out on Hypersil ODS2（4.6 mm×250 mm,5 μm）column with acetonitrile： 0.05% phosphoric acid-0.2% triethylamine-water（25∶75,V/V） as the mobile phase.The flow rate was 1.0 mL/min and the UV detection wavelength was 270 nm.Results indicated,Linearities of nuciferine salt was good in the ranges of 0.053～0.159 mg/mL（R2=0.999 2）.The average recovery was 96.4%,and RSD was 0.05%（n=6）.The method was simple,accurate,sensitivity and reproducible.The method can be used to the determination of nuciferine salt.