重组人碱性成纤维细胞生长因子对恒河猴眼激光视网膜损伤的治疗作用

Therapeutic Effect of Recombinant Human Basic Fibroblast Growth Factoron Laser Induced Retina Injuries in Rhesus Monkeys

目的探讨重组人碱性成纤维细胞生长因子（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｒｈｂＦＧＦ）对激光眼损伤的治疗作用。方法将采用氩离子激光照射（功率１８０～２７０ｍＷ）致视网膜损伤的恒河猴分４组，每组４只。其中２组动物自照射后１１～１２ｈ开始每３天１次分别球后注射ｒｈｂＦＧＦ１μｇ／ｋｇ和２μｇ／ｋｇ，１组动物于相同时间注射地塞米松１ｍｇ／ｋｇ作为阳性对照，另１组为照射对照组。激光照射后即刻和７、１４、２１、２８天行眼底彩色照相，记录激光损伤灶的形态变化，每天进行检眼镜观察。每组各有１只猴于照射后７、１４、２１、２８天行双眼球摘出术，分别作眼底组织学和超微结构观察。结果与地塞米松组和照射对照组比较，ｒｈｂＦＧＦ治疗组动物视网膜损伤斑明显变浅淡，色素沉着减少，视网膜损伤斑面积明显缩小（Ｐ＜００５），愈合修复较快。ｒｈｂＦＧＦ不仅能促进视网膜损伤区的细胞增生，而且能减少视细胞的损伤和缺失。结论ｒｈｂＦＧＦ对激光视网膜损伤有明显的治疗作用，有良好的临床应用前景。

Objective To study what effect rhbFGF has on laserinduced ratina injuries. Methods 16 rhesus monkeys whose retinas were injured by argon ion laser (180270 mW, exposed for 300 ms) were devided into four groups, 4 animals each. Two groups were injected postbulbarly with 1 g/kg and 2 g/kg rhbFGF, respectively, 1112 h after the monkeys were exposed to laser irradiation. The third group was treated with 1 mg/kg dexamethasone as positive control and the fouth group was the control group. Funduscopy was performed 0, 7, 14, 21 and 28 days after the procedure and the changes of the injured spots were recorded. Four monkeys from each groups were killed on 7, 14, 21 and 28 days after the treatments too and the eyes were used to make histological and ultrastructurral observation. Results rhbFGF can markedly reduce the retina pigmentation, lessen the extent of injury sopts ( P <005 ) and accelerate the healing and restoration process. It not only promotes cell proliferation in damaged retinal zones, but also abates cell loss and deletion. Conclusions rhbFGF has significant therapeutic effect on retinal injuries and it is hopeful for its clinical application.