关黄柏中生物碱类成分的“一测多评”

Quantitative analysis of multi-components with single marker for determination of alkaloids in Phellodendri Amurensis Cortex

目的建立同时测定关黄柏中小檗碱、巴马汀和药根碱的HPLC方法,在此基础上,建立"一测多评"法的方法学考察方式,验证该方法在关黄柏质量控制中应用的可行性和技术适用性。方法以关黄柏中主要成分小檗碱为指标,建立其与药根碱、巴马汀间的相对校正因子,并用该校正因子计算药根碱和巴马汀的量,对"一测多评"法的计算值与外标法实测值进行比较。结果建立了关黄柏中3种生物碱类成分"一测多评"法方法学的考察模式;"一测多评"法对不同的色谱柱和仪器的计算值与外标法实测值之间没有显著性差异。结论同时测定小檗碱、巴马汀和药根碱3种生物碱的"一测多评"法方法可靠,结果准确,可用于控制关黄柏药材及饮片的质量。

Objective To establish an HPLC method for simultaneously measuring the content of berberine,palmatine,and jatrorrhizine in Phellodendri Amurensis Cortex,and on this basis,the multiple components were examined by way of using a single test to validate the method’s feasibility and technical suitability of quality control applications for Phellodendri Amurensis Cortex.Methods Taking berberine,the main component in Phellodendri Amurensis Cortex,as an indicator,a relative correction factor between berberine and palmatine as well as jatrorrhizine was established.Then the correction factor was used to calculate the content of the other two in Phellodendri Amurensis Cortex.In this way,the calculated value was compared with the external standard method.Results A measuring method of evaluating the three alkaloids in Phellodendri Amurensis Cortex was established via using a single test;There was no significant difference compared with the external standard method.Conclusion The method for simultaneously measuring the content of berberine,palmatine,and jatrorrhizine in Phellodendri Amurensis Cortex is reliable and accurate,it could be used to control the quality of crude drugs and herbal pieces of Phellodendri Amurensis Cortex.