5种中药复方对局灶性脑缺血大鼠的影响及作用机制比较

Effects of five Chinese patent medicines on focal cerebral ischemia of rats and comparison on their mechanisms

目的比较通塞脉片、通心络胶囊、步长脑心通胶囊、复方血栓通胶囊、血塞通胶囊对局灶性脑缺血大鼠模型治疗作用的差异。方法采用大脑中动脉电凝法建立大鼠局灶性脑缺血模型,每天2次ig给予通塞脉片(0.239 g/kg)、通心络胶囊(0.281 g/kg)、步长脑心通胶囊(0.432 g/kg)、复方血栓通胶囊(0.405 g/kg)、血塞通胶囊(0.027 g/kg)治疗,连续给药3 d,观察5种药物对脑缺血大鼠行为学评分、脑梗死面积、血浆中血管紧张素I、II(AngI、AngII)及血清中C反应蛋白(CRP)及脑组织中核因子-κB(NF-κB)表达的影响。结果 5种中成药均可降低模型大鼠脑梗死面积,抑制NF-κB的表达,其中血塞通胶囊组大鼠行为学评分显著降低,通塞脉片组、通心络胶囊组、步长脑心通胶囊组和复方血栓通胶囊组大鼠血清IL-6的量显著降低,血塞通胶囊和步长脑心通胶囊组CRP的量显著降低,通塞脉片组大鼠血浆AngII的量显著降低(P<0.05、0.01)。结论 5种中成药均可不同程度地抑制炎症因子的释放,降低模型大鼠NF-κB的表达,从而改善脑缺血,另外通塞脉片还能通过降低AngII水平缓解缺血症状。

Objective To compare the difference of therapeutical effect with Tongsaimai Tablet,Tongxinluo Capsula,Buchang Naoxintong Capsula,Fufang Xueshuantong Capsula and Xuesaitong Capsula on focal cerebral ischemia of rats.Methods The focal cerebral ischemia of rats was made by electric coagulation with middle cerebral artery（MCA）.The score of ethology,the area of cerbral infarction,AngI,AngII in plasma,C-reactive protein（CRP） in serum,and NF-κB expression were examined to observe the effect of these five Chinese patent medicines by ig administration of Tongsaimai Tablet（0.239 g/kg）,Tongxinluo Capsula（0.281 g/kg）,Buchang Naoxintong Capsula（0.432 g/kg）,Fufang Xueshuantong Capsula（0.405 g/kg）,and Xuesaitong Capsula（0.027 g/kg） twice a day for 3 d.Results Every group of the five Chinese patent medicines could significantly reduce the area of cerebral infarction and the expression of NF-κB.The score of ethology was more lower with Xuesaitong Capsula.The content of IL-6 in plasma was cut down greatly by Tongxinluo Capsula,Buchang Naoxintong Capsula,Xuesaitong Capsula,and Tongsaimai Tablet.The content of CRP in serum was reduced with Xuesaitong Capsula and Buchang Naoxintong Capsula.The content of AngII in plasma was degraded by Tongsaimai Tablet（P〈0.05,0.01）.Conclusion Every Chinese patent medicine can inhibit the release of inflammatory factor at varying degrees.These five Chinese patent medicines can degrade the expression of NF-κB so as to ameliorate cerebral ischemia,and Tongsaimai Tablet can cut down the content of AngII in plasma to lessen the symptom of cerebral ischemia.