同时具有光敏感性和酶催化功能的复合敏感膜

Both light sensitivity and enzymatic activity of the composite sensitive film

采用溶液浇铸法成膜,把荧光指示剂和葡萄糖氧化物酶(GOD)同时固定于醋酸纤维素膜上,得到同时具有光敏感性和酶催化能力的复合敏感膜。利用SEM和紫外-可见光分光光度计对复合敏感膜表面形貌和酶活性进行了分析。荧光指示剂没有泄漏,固定化酶的稳定性高于游离酶,表明此醋酸纤维素膜可作为一种优良的荧光指示剂和酶固定化载体。

Cellulose acetate film was prepared by solution casting method.Composite sensing film can be obtained via immobilization of fluorescent indicator and glucose oxidase on cellulose acetate film.Surface morphology of composite sensitive film and enzymatic activity were analyzed by scanning electron microscope and UV-visible spectrometer.Fluorescent indicator does not leak.The stability of the immobilized enzyme was higher than the free enzyme.All above indicate this kind of cellulose acetate film can be a good immobilization carrier of fluorescent indicator and enzyme.