摘要
目的：对中药白芷的种质资源进行分析。方法：用１２个随机引物对野生白芷、杭白芷的４个居群超过１７个个体的基因组ＤＮＡ进行ＲＡＰＤ分析。结果：共扩增出４０个位点，其中多态位点２６个，占６．５％；祁、杭白芷的多态位点的百分率显示出低水平的遗传变异。根据ＲＡＰＤｉｓｔａｎｃｅ分析，祁、杭间的遗传距离（０．１６０）小于祁白芷居群内单株间和杭白芷不同居群间遗传距离，并且它们都小于野生白芷与祁、杭白芷间的遗传距离。结论：祁、杭白芷应属同一类群，与野生白芷有一定区别。
Objective:To analyse the germplasm in Baizhi.Method:Using 12 random primers,over seventeen indivi duals'RAPD were detected in four populations from wild Baizhi (Angelica dahurica) ,Qi Baizhi and Hang Baizhi.Results:A total of 40 loci were amplified,26 of which were polymorphism(65%).The PPS values of Hang Baizhi and Qi Baizhi indicate their low levels of genetic diversity.According to the RAPDinstance analysis,the genetic distance(0.16)between Qi Baizhi and Hang Baizhi is shorter than that of the individuals of the populations of Qi Baizhi and Hng Baizhi,and all of these genetic distances are shorter than those between wild Baizhi and Hang Baizhi(Qi Baizhi).Conclusion:Hang Baizhiand Qi Baizhi belong to the same taxon,which is different from the wild Baizhi.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1999年第8期457-459,共3页
China Journal of Chinese Materia Medica
基金
国家中医药管理局青年课题
国家自然科学基金资
关键词
野生白芷
祁白芷
杭白芷
RAPD
种质分析
wild Baizhi( Angelica dahurica )
Qi Baizhi
Hang Baizhi
RAPD
germplasm (original article on page 457)
