摘要
目的观察猪带绦虫虫卵实验感染中间宿主家猪后囊尾蚴致肝纤维化作用,并对病变程度进行定量分析。方法实验猪分成3组,将亚洲猪带绦虫虫卵以高剂量(60 000个/ml)、中剂量(40 000个/ml)和低剂量(10 000个/ml)分别灌服感染家猪,每种剂量感染2头。健康家猪2头为对照组。30 d后剖杀,取肝脏,制作病理切片,HE和Cason染色,对实验猪肝脏病变组织进行病理变化观察及胶原纤维含量分析。结果与健康对照组比较,实验组家猪肝组织发生不同程度的变性、坏死、炎细胞侵润,肝、脾指数增加(P<0.05或P<0.01);肝纤维化改变明显,胶原纤维总面积和总吸光度值增加,胶原纤维含量显著增加(P<0.05或P<0.01)。结论猪带绦虫虫卵感染家猪后以肝脏囊尾蚴寄生居多,同时发生以肉芽肿和纤维化改变为主的病理损害且较为严重。
Objective To observe liver fibrosis caused by experimentally infecting an intermediate host,i.e.pigs,with Taenia solium eggs and to quantitatively analyze the severity of that cysticercosis.Methods Three groups of pigs with 2 pigs each were directly fed a large dose of Asian Taenia solium eggs(60 000 eggs/ml),a medium dose,(40 000 eggs/ml),or a small dose(10 000 eggs/ml).Two healthy pigs served as the control group.Pigs were sacrificed after 30 days.The liver was removed and sectioned,and HE and Cason staining were performed.Pathological changes in tissue and collagen fiber content were analyzed.Results Compared to the control group,pigs fed large,medium,and small doses of eggs had varying degrees of liver tissue degeneration and necrosis,inflammatory cell infiltration,and an increased liver and spleen index(P〈0.05,P〈0.01).Liver fibrosis was apparent,the total area of collagen fibers and total optical density increased,and collagen fiber content also increased(P〈0.05,P〈0.01).Conclusion Pigs infected with Taenia solium eggs had a greater number of cysticerci in the liver and a greater incidence of pathological changes and more severe damage primarily in the form of granulomas and fibrosis.
出处
《中国病原生物学杂志》
CSCD
2011年第5期344-345,343,F0003,共4页
Journal of Pathogen Biology
基金
贵州省黔南州科学技术基金资助项目(No.2007008)
黔南民族医学高等专科学校科研基金资助项目(No.2007004)
关键词
猪带绦虫虫卵
实验感染
猪
肝纤维化
胶原纤维
Taenia solium eggs
experimental infection
pig
liver fibrosis
collagen fibers