矾冰纳米乳对L-929细胞抑制作用的影响

The Inhibition Effect of Nano Size Alum-Borneol Emulsion to L-929 Cell

目的:探讨矾冰纳米乳对L-929实验细胞增殖抑制作用。方法:采用细胞病变抑制法与MTT法,将试验药物稀释后加入培养4h贴壁的L-929细胞中,37℃、5%CO2培养箱中观察24h、48h,同时设传统药物矾冰液和正常细胞对照,倒置显微镜下观察药物对细胞形态的影响,测定OD值比较药物对细胞生长的影响。结果:矾冰纳米乳对L-929细胞的生长有随浓度增加其生长抑制率也增加的趋势,与矾冰液比较有统计学差异(P<0.05);矾冰纳米乳在同一浓度、同一时间内细胞增长抑制率低于矾冰液,差异有统计学意义(P<0.05);同时矾冰纳米乳有随浓度降低,细胞培养时间延长其细胞抑制率也降低,与正常组细胞比较无统计学差异(P>0.05);倒置显微镜下观察细胞形态良好。结论:纳米矾冰乳对L-929培养细胞无毒性作用,可以用于临床。

Objective：To study the proliferative inhibition effect of nano size Alum-Borneol emulsion to L-929 cell.Methods：The cytopathogenic effect inhibition assay and MTT method were applied.The adherent L-929 cell were added diluted drug and cultrued 24h,48h in 7℃,5% CO2 condition.The normal cell group and traditional Alum-Borneol liquid group were set as control.The effect of drugs to cell morphology and growth were detected by inverted microscope and OD assay respectively.Results：Compared with traditional Alum-Borneol liquid group,the nano size Alum-Borneol emulsion could increase the proliferative inhibition effect on L-929 cell with increasing of drug concentration（P0.05）.The proliferative inhibition rate of nano size Alum-Borneol emulsion was lower than traditional Alum-Borneol liquid at same time and concentration（P0.05）.The proliferative inhibition rate of nano size Alum-Borneol emulsion was decreased with decreasing drug concentration and cultural time compared with normal control group（P0.05）.The morphology results show a good cell shape in inverted microscope.Conclusion：The nano size Alum-Borneol emulsion has no cytotoxicity to L-929 cell and could applied to clinic.