超声-酸性铬酸钡分光光度法测定中药饮片中掺杂的硫酸盐

Determination of Sulfate Adulterated in Decoction Pieces by Ultrasonic-acidic Barium Chromate Spectrophotometry

目的：建立简便易行的硫酸盐定量分析方法,以便药检单位有效控制中药饮片的质量。方法：以穿山甲和石斛为动物药和植物药样本代表,进行紫外分光光度测定法的建立,并将该方法广泛用于20多种含硫酸盐中药饮片的测定。结果：硫酸盐在5.0~250.0μg范围内线性关系良好（r=0.9994）;穿山甲硫酸盐含量为78.1mg.g-1,平均回收率（n=9）为99.2%;石斛硫酸盐含量为120.7mg.g-1,平均回收率（n=9）为98.8%。结论：本测定方法简便、快速,准确性和重复性好,具有推广于其它药检所的前景。

Objective：To develop a convenient method for determining the quantitative of Sulfate Adulterated in decoction pieces so as to control the quality.Methods：ChuanShanJia and ShiHu were selected as represents of different samples to establish the Spectrophotometry,meanwhile,the method was widely used in more than 20 decoction pieces.Results：The linear range of sulfate was 5.0~250.0μg（r=0.9994）;The quantitative of sulfate adulterated in ChuanShanJia was 78.1 mg·g-1,The average recovery（n=9）was 99.2%;The quantitative of sulfate adulterated in ShiHu was 120.7 mg·g-1,The average recovery（n=9）was 98.8%.Concusion：The method is simple,accurate and reliable for determination of sulfate adulterated in decoction pieces.It seems that the method has prospect to be manipulated by other drug control institutes.