HE染色切片褪色后免疫组化染色方法研究

Explore the Method of Immunohistochemiscal Staining for HE Slides after Decoloration

目的探讨HE染色切片褪色后免疫组织化学染色方法。方法 HE染片放入二甲苯中浸泡,清除盖玻片和树胶;梯度酒精水化,蒸馏水洗涤;置入0.25%高锰酸钾5min,蒸馏水洗,40.5%草酸溶液漂白,蒸馏水洗,自然晾干;滴PLLS 1滴于组织片上,摇匀,将切片在60℃烘箱中烘60min烘干;PBS洗5min×3次;组织需要进行水浴抗原热修复;与对照片按标准S-P方法进行免疫组化染色。结果所有对照片与试验片均为阳性,无一例脱片。结论此方法可用于HE片褪色做免疫组化染色,具有实用价值。

Objective To explore the method of immunohistochemiscal staining for HE slides after decoloration. Methods Dip HE slides in xylene to remove coverslip and glues; Rehydrate in alcohol gradient, rinse the slides in deionized water; Placed in 0.25% potassium permanganate for five minutes, rinse the slides in deionized water, bleached with 40.5% oxalic acid, rinse the slides in deionized water, dry the slides at room temperature; Drip one drop of PLLS on the tissue slides and bake to dry in oven at 60℃ about 60 minutes; Rinse slides three times in deionized water for 5 minutes; Use water-bath heating for antigen retrieval; Both control slides and tested slides use standard S-P method to have immunohistochemiscal staining. Results Positive staining were observed in both control slides and tested slides and all slides in good condition. Conclusion The method tested in this study is applicable for immunohistochemical staining in HE slides after decoloration.