大豆毛状根-VA菌根真菌双重培养体系的建立

Establishment of Dual Culture System of Soybean [Glycine max(L.) Merr] Hairy Roots and Vesicular-Arbuscular(VA) Mycorrhizal Fungi

以大豆毛状根为宿主,接种VA菌根真菌珠状巨孢囊霉(Gigaspora margarita),经过3.5个月的双重培养,观察到VA菌根真菌珠状巨孢囊霉对大豆毛状根的侵染,辅助细胞形成,并获得VA菌根真菌成熟孢子,在无菌条件下建立了大豆毛状根-VA菌根真菌双重培养体系,为研究菌根真菌侵染大豆根部形成共生体系及相关分子机制提供了一种有效的研究方法。

This paper describes the processes of establishment of dual culture system of soybean hairy roots and vesicular-arbuscular（VA） mycorrhizal fungi Gigaspora margarita under axenic conditions.Soybean hairy roots were used as host to be infected by G.margarita.After 3.5 months of dual culture,the infection of transformed soybean hairy roots by VA mycorrhizal fungi was observed,subsequently auxiliary cells were formed and mature spores of G.margarita were obtained.The successful establishment of dual culture system of soybean and VA mycorrhizal fungi provides an effective method to study the mechanisms of symbiosis between soybean roots and VA mycorrhizal fungi and related molecular researches.