摘要
利用GenomeLab(tm)GeXP遗传分析系统建立一种同时检测A组轮状病毒、诺如病毒GI、GII型、札如病毒、肠道腺病毒、星状病毒、人博卡病毒II型7种常见腹泻相关病毒的方法。对反应条件进行优化后,非同日三次重复实验表明至少在104拷贝/μL水平可同时特异地检测出7种病毒,对Enterovirus71、Human Parechovirus、Picobir-navirusII阳性标本无交叉反应。本研究初步建立了一种高通量、快速的常见腹泻相关病毒的检测方法,为腹泻病原的分子诊断提供了新的方法。
To develop and optimize a simultaneous detection method of RotavirusA,Norovirus GI,GII,Sapovirus,human astrovirus,enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system.The sensitivity was verified to be 104 copies/μL with plasmids containing the viral targets in triplicate on different days,and no cross-reaction with enterovirus71,human Parechovirus and PicobirnavirusII was observed.Finally,we successfully developed a high throughout,rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis,which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
出处
《病毒学报》
CAS
CSCD
北大核心
2011年第3期288-293,共6页
Chinese Journal of Virology
基金
传染病防治重大专项(2009ZX10004-101
2008ZX10004-004)
