转基因食品DNA提取研究进展

Progress of the Study on the Method of DNA Extraction from Genetically Modified Food

为了满足消费者对转基因食品的知情权,建立准确、快速、高效的转基因成分检测技术至关重要,而高质量DNA模板的获取,是转基因食品进行基因检测的前提。对近几年来国内外转基因食品DNA提取方法:十六烷基三甲基溴化铵(hexadecyl trimethyl ammonium bromide,CTAB)法、十二烷基硫酸钠(dodecyl sulfate,sodium salt,SDS)法、聚乙烯吡咯烷酮(polyvinylpyrrolidone,PVP)法、介质纯化法、螯合树脂法等进行了介绍,对各种方法进行综合评述并重点阐述了提取中应注意的问题和转基因食品DNA提取的发展方向,为初步进行DNA提取的科学研究者提供有效的信息。

In order to meet consumers’ right to know for genetically modified（GM） food,it is very important to establish accurate,rapid and efficient GM food detection technology.The quality and quantity of the DNA is considered to be a prerequisite factor for the GM food detection.Several DNA extraction methods used at home and abroad in the recent years are reviewed,such as hexadecyl trimethyl ammonium bromide（CTAB） methods,dodecyl sulfate,sodium salt（SDS） methods,polyvinylpyrrolidone（PVP）methods,medium purify methods,chelating resin methods etc.The advantages,disadvantages,application range of the above methods are comprehensively summarized,along with analyzes the problems should be paid attention to and the GM food DNA extraction developing direction,which will offer operative information for rudimenting workers in DNA extraction.