山豆根多糖硫酸化修饰及其体外抗HBV活性测定

Sulfated modification for sophora subprostrate polysaccharide and anti-HBV activity determination of the modifiers

目的对山豆根多糖进行硫酸化修饰并对其抗HBV活性进行测定。方法提取山豆根多糖,采用氯磺酸-吡啶法对山豆根多糖进行硫酸化修饰,产物经红外光谱鉴定。用MTT法测定该修饰物的抗HBV活性。结果通过山豆根多糖硫酸化前后的红外图谱分析,表明硫酸基与多糖已结合成酯,并且有抗HBV的作用。结论山豆根多糖硫酸化修饰能提高对乙型肝炎病毒细胞转染的2215细胞培养液中HBeAg、HBsAg的抑制作用。

AIM To modify sophom subprostrate polysaeeharide（SSP） of the sulfated, and to determine anti-HBV activity. METHODS SSP was extract from sophora subprostrate , which was modified by chlorosulfonic acid-pyridine method. Products were identified by infrared spectnnn（IR）. The anti-HBV activity of modification production was tested by MTF method. RESULTS Analysis of IR spectrum for determination of SSP before and after modification showed that sulfate and polysaccharides have been combined into esters,and they had anti-HBV effect. CONCLUSION The experiments in vitro for determining anti-HBV activity show that modification production can enhance inhibitory action of HbeAg,HBsAg to 2 215 cells culture medium transfected by HBV.