抑癌基因p53在胃癌组织的表达及临床意义

Clinical Implication of p53 Gene Expression in Human Gastric Cancer

目的 :探讨 p5 3基因在胃癌的表达与临床、病理因素及预后的关系 ,探索 p5 3基因异常表达在胃癌发生过程中的作用。方法 :使用抗p5 3蛋白的单克隆抗体DO - 7,对 98例人胃癌组织 p5 3的表达进行免疫组化研究。 结果 :48例胃癌组织 p5 3基因表达阳性 (49% )。p5 3基因表达与胃癌的大体类型、分化程度、生长方式、浸润深度、淋巴结转移等指标均无明显关系。在 10例有不典型增生的癌旁组织中 ,2例呈阳性反应 ;在 4例有肠上皮化生的癌旁组织中及其余正常的癌旁组织中均无阳性表达。根据有随访资料的 5 5例统计结果 ,显示 p5 3表达与胃癌预后无显著性相关 (P>0 .0 5 )。结论 :p5 3基因的突变可能是胃癌发生发展过程中的早期事件。由于 p5 3表达在胃癌组织有较高的阳性率 ,其测定可用于对高危人群的筛选。

Objective: To probe the relationship between the expression of p53 gene in gastric cancer and its clinical implication, pathogenesis and prognosis. Methods: The p53 gene in 98 gastric cancer tissue specimens were determined by immunohistochemical staining. Results: The p53 gene expression showed positive in 48 specimens (49%). p53 gene was not correlated with the gross type, histologic differentiation, growth pattern, depth of invasion and lymph node metastasis of gastric cancer. In 10 specimens of pericancerous tissue with atypical dysplasia, 2 of them showed positive expression, but in 4 specimens of pericancerous tissue with intestinal metaplasia and other normal tissues adjacent to the tumor, the p53 gene expressions were all negative. Follow-up study showed that there was no correlation between p53 expression and prognosis of gastric cancer ( P >0.05). Conclusion: The results indicate that mutation of p53 gene may be an early event in the development of gastric cancer, and that it bears no relation with the process of invasion and metastasis of GC. Thus, assay of p53 gene expression in biopsy specimens might be useful in screening of high-risk populations.