β-榄香烯诱发肿瘤细胞凋亡的研究

The Antitumor Activity of β-elemene Associates with Apoptosis

目的 :探讨 β -榄香烯的抗癌疗效及其作用机制。方法 :采用MTT方法、Hoechst3334 2和PI荧光染色法、电镜及流式细胞术分析法 ,发现 β-榄香烯乳剂对K562 细胞生长的影响。结果 :β -榄香烯明显抑制K562 细胞的生长 ,对K562 细胞的半数生长抑制剂量 (IC50 )为 17.14μg/ml。其抑制细胞生长能力主要是通过诱导细胞凋亡 ,并且呈浓度和时间依赖性。在影响细胞周期方面 ,主要使G1期细胞数目增多 ,S期细胞数目下降。结论 :β -榄香烯诱导细胞凋亡是其抗肿瘤作用机制的重要方面之一。

elemene is an element isolated from the Chinese medical herb Rhizomae. The effect of β-elemene on the growth of K 562 cells was evaluated by MTT Hoechst 33342 staining, PI staining and electromicroscopy as well as Flow Cytometry assay. The result showed that β-elemene significantly inhibited K 562 cells growth and that the IC50 values of β-elemene for K 562 cells was found to be 17.14 ug/ml. Its induction ratio of apoptosis was positively related with the concentration and the exposure time to β-elemene. The number of phase G 1 cell increased and S cell decreased which indicated that the induction of apoptosis was ascribed to mechanisms of antitumor activity of β-elemene.