EZH2基因与卵巢癌细胞系A2780化疗敏感性的关系

The Relationship of EZH2 Gene and Chemosensibility of Ovarian Cancer Cell Line A2780

目的:探讨RNA干扰沉默果蝇zeste基因增强子同源物2(enhancer of zeste homolog2,EZH2)基因与卵巢癌细胞A2780顺铂敏感性的关系。方法:获得稳定表达EZH2短发夹状RNA(short hairpin RNA,shRNA)的人卵巢癌细胞A2780-shEZH2,实时定量PCR(Real time RT-PCR)法和免疫印记法检测转染株的EZH2基因表达,MTT法检测细胞对顺铂的耐药性,流式细胞学检测细胞凋亡。结果:以未转染组A2780细胞EZH2的表达水平为100%,EZH2-shRNA转染组A2780-shEZH2细胞EZH2的mRNA及蛋白水平分别下降90.20±1.66%和76.54±8.92%,与未转染组比较差异均有高度统计学意义(P<0.01)。EZH2-shRNA转染组A2780-shEZH2细胞对顺铂的IC50与未转染组A2780细胞IC50值和空质粒转染组A2780-shVector细胞IC50值比较,差异无统计学意义(P>0.05)。EZH2-shEZH2转染组的细胞凋亡率与空质粒转染组凋亡率及未转染细胞凋亡率相比较,差异无统计学意义(P>0.05)。结论:EZH2基因与卵巢癌原发性顺铂耐药无关。

Objective：To investigate the relationship of RNA interfering EZH2 and cisplatin sensibility of ovarian cancer cell line A2780.Methods： After the EZH2-knockdown A2780 cell lines by short hair pin RNA technique（shRNA） were obtained,the mRNA and protein expression of EZH2 were detected by real time RT-PCR and western blotting.The 50% inhibition concentration（IC50） of cisplatin was determined by MTT assays,and the cell apoptosis was evaluated by flow cytometry.Results：After the transfection of EZH2 shRNA into A2780,the expressions of mRNA and protein of EZH2 gene decreased by（90.20±1.66）% and（76.54±8.92）% respectively,and the differences were significant when compared with non-transfection group（P0.01）.No differences were found in cisplatin IC50 and cell apoptosis among EZH2-shRNA transfection group,empty vector transfection group and non-transfection group（P0.05）.Conclusions： EZH2 gene plays little role in primary cisplatin resistance of ovarian cancer.