赣州市569份HIV抗体初筛阳性标本复检与确证结果分析

The Analysis of Re-inspection and Confirmation for 569 HIV-Antibody Preliminary Screening Positive Samples of Ganzhou

目的了解赣州市不同人群HIV抗体感染状况，探讨HIV抗体初筛、复检与确证实验的准确性，为指导与提高各筛查实验室的检测水平提供依据。方法对2002年1月-2009年12月该市艾滋病筛查实验室经酶联免疫吸附试验初筛阳性送检样本569份，采用两种ELISA试剂和金标法进行复检，复检阳性可疑标本进行免疫印迹法（WB）确证检测，并对其结果进行回顾性分析。结果569份初筛阳性标本复检总符合率为38．84％（221／569），确证阳性符合率为68．33％（151／221）。确证阴性59份，不确定11份。不确定中出现gp1604-p24条带5份；p24单条带3份；gpl60和p17单条带各1份；p39．4-p24条带1份。ELISA法复检符合率为61．54％（24／39），确证阳性符合率50．00％（12／24）。ELISA法和金标法复检符合率为34．34％（182／530），确证阳性符合率为98．58％（139／141）。在确证的151份H1V阳性感染者中，男性100份（占66．23％），女性51份（占33．77％），两者差异有统计学意义（P〈0．01）。HIV阳性感染者有年龄增长趋势，多为40-和50-年龄段。结论采用ELISA加金标试剂复核，可剔除筛查实验室假阳性标本，提高确证的阳性符合率，降低检测成本。

Objective To understand the infected facts of HIV antibodies in different Ganzhou populations, and survey the accuracy of the preliminary screening, re-inspection and confirmation experiments on HIV antibody, to offer an accordance for guiding and improving the examination level of the screening and in- spection laboratories. Methods Using two ELISA and .1B-P reagent to re-inspect 569 preliminarily- screened positive samples which submit to Ganzhou city AIDS screening and inspection laboratories from Jan. 2002 to Dec. 2009, then to confirm the suspicious re-examination positive specimens by the method of Western blot （ WB）. Results The total compliance re-inspection rate was 38.84% （221/569） and the confirmed positive coincidence rate was 68.33 % （ 151/221 ）. Fifty-nine were confirmed negative, and 11 uncertain（ 5 gpl60 ＋ p24 bands, 3 p24 bands and each one of gpl60, p17 and p39 ＋ p24 band were positive）. The re-examination compliance rate and the confirmed positive coincidence rate of ELISA method was 61.54% （24/39） and 50.00% （12/24）. The re-examination compliance rate and the confirmed positive coincidence rate of ELISA and JB-P method was 34.34% （182/530） and 98.58% （ 139/141 ）. In 151 cases who was confirmed HIV-positive, male were 100 （66.23%） and female were 51 （33.77%）. There was a statistical significance between them. HIV-positive infected trend with age, mostly 40 and 50 age groups. Conclusion Re-inspection experiment with ELISA and JB-P reagent can increase the confirmed positive coincidence rate, eliminate the false positive samples in screening and inspection laboratories, and also cut the cost in the experiment.