HIV阳性患者血培养法分离马尔尼菲青霉菌

The Isolated Method of Penicillium Marneffei were Isolated from Blood Culture of HIV Positive Cases

目的探讨如何利用血培养法从HIV阳性患者血液标本检测马尔尼菲青霉菌。方法从两个不同部位分别无菌抽取患者血液于2个BD需氧血培养瓶中,用BD9240全自动血培养仪进行培养。仪器阳性报警后,若涂片见到真菌孢子或菌丝,初代培养用沙氏琼脂培养基及血平板35℃培养。对于丝状菌落适时转种2块沙氏琼脂培养基,分别进行35℃和25℃培养。每日观察菌落及菌体形态变化情况,用乳酸酚棉蓝染色法观察菌体形态。结果从63例HIV阳性患者送检的血培养中检测出6株马尔尼菲青霉菌。结论采用血培养法可以从HIV患者的血液标本中检测出马尔尼菲青霉菌,细菌室应提高检测能力,及时准确检测出马尔尼菲青霉菌。

Objective To evaluate the method of blood culture to isolate Penicillium marneffei from HIV patients. Methods Blood from two different parts of patients were put into two BD aerobic blood culture bottles, cultured by BD 9240 instrument. If fungal spores or hypha on the smear were found, the instrument would alarm and blood samples were initially cultured with Sabouraud agar medium and blood plate respectively at 35℃ and 25℃ When filamentous colonies formed in due course, they were cultured in two kinds of Sab- ouraud agar medium, and observed daily on colony morphology changes with lactic acid phenolic cotton blue staining. Results There were 6 strains of Penicillium marneffei isolated from 63 cases of HIV patients with blood culture. Conclusion HIV Patients were easily infected with Penicillium marneffei and bacteriology laboratory should be alert to run for the timely detection of Penicillium marneffe.