雌激素对人乳腺癌细胞系基质细胞衍化因子-1影响的观察

Effects of estrogen on the expression of SDF-1 in human breast cancer cell lines

目的:探讨雌激素对基质细胞衍化因子-1(SDF-1)的影响。方法:选取乳腺癌细胞系MCF-7和MDA-MB-231为研究对象,分成对照组、雌激素组和雌激素+雌激素受体阻断组,分别加入不同生理浓度的17-β雌二醇作用一定时间以及同一浓度17-β雌二醇作用不同时间点,用酶联免疫吸附实验(ELISA)方法测定培养液中SDF-1的浓度,半定量逆转录聚合酶链反应(RT-PCR)法检测细胞SDF-1 mRNA的表达。结果:MDA-MB-231细胞系加与未加雌激素,均未检测到SDF-1的分泌。而MCF-7细胞基础培养液中可检测到SDF-1分泌。不同生理浓度的17-β雌二醇均可增加MCF-7细胞SDF-1的分泌水平。当加入1×10-7mol/L的生理高浓度17-β雌二醇时,细胞分泌SDF-1水平在2 h达到高峰,是对照组的6倍〔(1 823.17±325.18)ρg/mLvs(307.23±5.42)ρg/mL,F=201.02,P<0.01〕,该作用可被雌激素受体拮抗剂(ICI182,780)消除。此外,SDF-1mRNA的表达水平与测得的SDF-1蛋白水平相一致。结论:在某些乳腺癌细胞系,生理浓度的雌激素可促进SDF-1的分泌,而这种作用主要是通过雌激素受体来实现。雌激素可通过SDF-1来影响乳腺癌的生物学特性。

OBJECTIVE： To investigate the effect of estrogen on the biological characteristics of breast cancer cells through SDF-1. METHODS：Two breast cancer cell lines（MCF-7 and MDA- MB-231） were used for tests, which were divided into control group, estrogen group and estrogen plus estrogen receptor blocker group. 17-β estradiol of different physiological concentrations was applied to the cultured cells in the three groups at a setted of time. The SDF-1 level in culture medium was measured by enzyme linked immunosorbent assay （ELISA） and the expression of SDF-1 mRNA was examined using semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） technique. RESULTS：SDF-1 protein was not detected in MDA-MB-231 cell line with or without estrogen, however, SDF-1 protein could be detected in MCF-7 cell line, and the level of SDF-1 increased with the application of 17-β estradiol applied to the cells. When adding 1 × 10^-7 mol/L of physiological concentration of 17-β estradiol to the culture cells for 2 h, the SDF-1 levels reached a peak, which was 6 times than the control group [（1 823. 17±325. 18） ρg/mL vs （307. 23± 5. 42）ρg/mL, F=201. 02, P〈0. 01]. Furthermore, the secretion of SDF-1 increased by estradiol could be eliminated by a pure estrogen receptor antagonist （ICI182, 780）. In addition, SDF-1 mRNA expression level was also changed in a similar way as SDF-1 protein. CONCLUSIONS：In some breast cancer cell lines, physiological concentrations of estrogen can increase the secretion of SDF-1, and this effect is mainly achieved through estrogen combined with its recep tor. Estrogen can affect the biological behaviour of breast cancer cells by SDF-1.