siRNA干扰XIAP基因对卵巢癌SKOV-3细胞生物学功能影响的研究

Effects of interference of XIAP by siRNA on the biological behaviors in ovarian carcinoma SKOV-3 cells

目的:采用小分子干扰RNA(siR-NA)技术沉默卵巢癌SKOV-3细胞X连锁凋亡抑制蛋白(XIAP)基因的表达,观察其对卵巢癌细胞增殖能力和凋亡的影响。方法:将XIAP siRNA转染SKOV-3细胞,MTT检测细胞增殖抑制情况,Annexin-V检测细胞凋亡率的变化,FCM法检测细胞周期变化。结果:XIAP siRNA转染后能明显抑制XIAP蛋白的表达,与其他组相比差异有统计学意义,F=63.782,P<0.05;特异性干涉组细胞的增殖能力明显低于空白对照组及非特异性干涉组,F=53.412,P<0.05;XIAP-siRNA转染组的细胞凋亡率为〔(31.54±0.62)%〕,与非特异性干涉组〔(5.34±0.38)%〕和空白对照组〔(6.73±0.46)%〕相比,差异有统计学意义,F=102.32,P<0.01。SKOV-3细胞明显阻滞在G0/G1期,与其他组相比,差异有统计学意义,P<0.01。结论:干扰XIAP基因可抑制卵巢癌SKOV-3细胞的增殖,诱导其凋亡。

OBJECTIVE： To silence the expression of XIAP in ovarian cancer cell line SKOV3 by using small interfering RNA （siRNA）, and observe its effects on the proliferation and apoptosis. METHODS： The XIAP siRNA was transfected into SKOV-3 cells and the cell proliferation was evaluated using MTT assay. The apoptosis rate and cell cycle distribution were analyzed using Annexin-V and flow cytometry methods. RESULTS： XIAP siRNA significantly suppressed the expression of XIAP gene in SKOV-3 cells. The difference was significant com- pared with other groups （F=63. 782,P〈0.05）. Specific group cells were slower than normal and nonspecific groups in the rate of cell proliferation （F 53. 412, P%0.05）. The apoptosis percentage of interference group [（31. 54 ± 0. 62） %] was higher than that of nonspecific [（5. 34 ± 0. 38） %] and control groups （（6.73±0.46）~,（F=102.32, P〈0.01）]. After transfection XIAP-siRNA,SKOV-3 cells were arrested at G0/G1 phase. The difference was significant compared with other groups （P〈0.01 ）. CONCLUSION： Silencing XIAP gene can inhibite the proliferation and induced the apoptosis of SKOV-3 cells.