摘要
目的观察Th1型和Th2型细胞因子在口腔扁平苔藓(OLP)外周血中的表达。方法密度梯度离心法分离20例充血糜烂型,16例光滑型OLP病例和19例正常对照组人群的外周血单个核细胞,逆转录-聚合酶链反应法检测各组外周血单个核细胞中IFN-gamma mRNA和IL-4 mRNA的表达;ELISA法检测各组血清中IFN-gamma和IL-4的表达。结果充血糜烂型和光滑型OLP患者中IL-4 mRNA和蛋白的表达均高于正常对照组,差异有统计学意义(P<0.01);而充血糜烂型和光滑型OLP患者中IFN-gamma mRNA和蛋白的表达均低于正常对照组,差异有统计学意义(P<0.01)。但表达结果在充血糜烂型及光滑型OLP患者组间的差异无统计学意义(P>0.05)。结论 Th1/Th2失衡表达与OLP发病机制密切相关,为临床治疗OLP提供新的思路。
Objective To observe the expression of Th1/Th2 in peripheral blood of oral lichen planus（OLP） and to further explore the pathogenesis of OLP.Methods The expression of IFN-gamma mRNA（typical Th1 cytokine）,IL-4 mRNA（typical Th2 cytokine） was investigated in peripheral blood mononuclear cell（PBMC） seperated from erythematous/erosive OLP（20 cases）,reticular OLP（16cases） and normal controls（19 cases） using RT-PCR methods;and IFN-gamma,IL-4 in serum was tested by ELISA.Results RT-PCR results showed that expression of IL-4 mRNA is higher in erythematous/erosive OLP and reticular OLP than that in control group（P 0.01）;In contrast,expression of IFN-gamma mRNA was lower in erythematous/erosive OLP and reticular OLP than that control group（P 0.01）.ELISA assay indicated that erythematous/erosive and reticular OLP patients had a higher expression level of IL-4 protein and lower expression level of IFN-gamma protein in serum than normal controls（P0.01）.However,there were no significant differences between erythematous/erosive and reticular groups（P0.05）.Conclusions The data accumulated here strongly indicate that unbalanced expression of Th1/Th2 was involved in the pathogenesis of OLP,and provide new ideas for clinical treatment of OLP.
出处
《口腔医学》
CAS
2011年第5期291-293,308,共4页
Stomatology
基金
南京医科大学科技发展基金重点项目(06NMUZO22)
