氟康唑对HIV感染者口腔白色念珠菌抗真菌后效应的体外研究

In vitro Post-antifungal Effect of Fluconazole against Oral Candida Albicans Isolates in HIV Disease

目的评估氟康唑体外最小抑菌浓度(minimum inhibitory concentration,MIC)和检测暴露于小剂量氟康唑后白色念珠菌口腔分离株的抗真菌后效应(post-antifungal effect,PAFE)。方法将白色念珠菌标准株和10株临床分离株分别暴露于4×MIC和6×MIC的氟康唑后,使用自动化的浊度分析测量法检测白色念珠菌PAFE的存在。结果由4×MIC和6×MIC的氟康唑在RPMI 1640液体培养基中诱导的白色念珠菌标准株产生的PAFE平均持续时间分别为0.12 h和3.14 h;诱导的10株临床分离株产生的PAFE持续时间平均分别为(0.19±0.24)h和(2.08±0.25)h。其中6×MIC氟康唑的PAFE在临床菌株内差异有统计学意义(P=0.000),而4×MIC氟康唑的PAFE在临床菌株内差异没有统计学意义(P=0.529)。结论氟康唑能有效地抑制白色念珠菌的生长,在白色念珠菌中引起PAFE。

Objective To evaluate the minimum inhibitory concentration （MIC） and measure post-antifungal effect （PAFE） of oral Candida albicans isolates following limited exposure to flueonazole. Methods Ten oral isolates of C. albicans were examined for the presence of the PAFE following limited exposure to fluconazole （4 x MIC and 6 x MIC）, respectively, measured using automated turbidometry. Results The duration of the mean PAFE for C. albican ATCC 90031 induced by fluconazole at concentrations of 4 x MIC and 6 x MIC in RPMI medium were 0.12 h and 3.14 h respec- tively, compared with （0.19 ± O. 24） h and （2.08 ± 0.25 ） h respectively, for all 10 isolates of C. albicans. Significant intra-strain differences in PAFE were observed after exposure to 6 x MIC flueonazole （ P = O. 000）, but not for 4 x MIC fluconazole （ P = 0. 529 ）. Conclusion Flueonazole can effectively perturb C. albicans growth and elicited a peired of PAFE in C. albicans.