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老年大鼠胼胝体额区有髓神经纤维与髓鞘改变的研究 被引量：1

Myelin in the frontal region of corpus callosum in old rats
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摘要 目的:探讨老年大鼠胼胝体额区有髓神经纤维、髓鞘的结构变化和髓鞘碱性蛋白(Myelin basic protein,MBP)的表达变化,以及二者间的关系。方法:运用透射电子显微镜和新的体视学方法分别对年轻组和老年组雌性Long-Evans大鼠胼胝体额区有髓神经纤维和髓鞘进行定量研究,运用Western blot对年轻组和老年组大鼠胼胝体额区MBP表达进行定量研究。结果:与年轻组相比,老年组胼胝体额区体积存在显著性降低,有髓神经纤维总体积显著性下降,21.5 kD MBP表达量显著性降低。21.5 kD MBP的表达降低与有髓神经纤维总体积、髓鞘总体积的降低之间呈正相关。结论:胼胝体存在显著性老年萎缩,胼胝体额区有髓神经纤维和MBP的老年改变可能与老年大脑额叶功能下降有关。 Objective： To explore the age-related changes of the myelinated fibers, myelin sheath and the expression of myelin basicprotein in the frontal region of the corpus callosum in old rats, and their relationship. Methods： The myelinated fibers and sheathes in the frontal region of corpus callosum of young and old-aged female Long-Evans rats were quantitatively studied using electron microscope and unbiased stereological techniques.Myelin basic proteins in the frontal region of corpus callosum of young andold-aged female rats were quantitatively studied using Western blot. Results： When compared with young female rats, the total volume of the frontal region of the corpus callosum and the total volume of the myelinated fibers in the frontal region of old corpus callosum were significantly degraded. The expression of 21.5 kD MBP was significantly decreased in old rats. The reduction of the expression of 21.5 kD MBP, the reduction of the total volume of the myelinated fibers and the total volume of the myelin sheathes were positively correlated. Conclusion： There is age-related atrophy of the frontal region of corpus callosum. The age-related changes of the myelinated fibers and MBP in the frontal region of corpus callosum may be related to the decline of frontal lobe functions with aging.
作者 徐瑲 兰欢 卢伟 唐勇
机构地区 泸州医学院组织胚胎学教研室 泸州医学院心肌电生理学研究室 重庆医科大学基础医学院组织胚胎学教研室
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2011年第4期440-443,共4页 Journal of Chongqing Medical University
基金 国家自然科学基金资助项目(编号:30572075) 重庆市自然科学基金资助项目(编号:2005BB5034) 泸州医学院青年基金资助项目(编号:201017)
关键词 胼胝体额区 髓鞘碱性蛋白 有髓神经纤维 体视学 老年改变 frontal region of corpus callosum myelin basic protein myelinated fibers stereology age-related change
分类号 R322.81 [医药卫生—人体解剖和组织胚胎学] R329.54 [医药卫生—人体解剖和组织胚胎学]
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参考文献21

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二级参考文献22

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共引文献19

同被引文献14

  • 1李继硕.神经科学基础[M].北京:高等教育出社,2003.69,227.
  • 2蒋文华.神经解剖学[M].上海:复旦大学出版社,2004,316-336.
  • 3Bellis TJ, Wilber LA. Effects of aging and gender on interhemispheric function[J]. Speech Language Hearing kes,2001, 44(2): 246-263.
  • 4Ballmaier M, Kumae A, Elderkin-Thompson V, et al. Mapping callosal morphology in early- and late-onset elderly depression: an index of distinct changes in cortical connectivity [J]. Neuropsychopharmacology,2008, 33(7): 1528-1536.
  • 5Ota M, Obata T, Akine Y, et al. Age-related degeneration of corpus callosum measured with diffusion tensor imaging[J] .Neuroimage, 2006 Jul 15,31 (4) : 1445- 1452.
  • 6Sullivan EV, Adabteinsson E, Pfefferbaum A, et al. Selective age-related degradation of anterior callosal fiber bundles quantified in vivo with fiber tracking [J]. Cereb Cortex, 2006,16:1030-1039.
  • 7Hernandez A, Fox GR, Ben-Shachar M, et al. Development of the human corpus caUosum [C]. 2006 Neuroscience Meeting Planner. Atlanta, GA: Society of Ncuroscience, 2006.
  • 8Tang Y, Nyengaard JR, Pakkenberg B, et al. Age- induced white matter changes in the human brain: A stereological investigation [J]. Neurobiol Aging, 1997, 18 (6): 609-615.
  • 9Liddell B, Paul R, Arns M, et al. Rates of decline distinguish Alzheimer's disease and mild cognitive impairment relative to normal aging: integrating cognition and brain function[J]. Integr Neurosci,2007, 6(1): 141-174.
  • 10Qiang X, Wei L, Xuan Q, et al. Age related changes in the myelinated fibers of corpus callosum [J]. Neuroscience Letters,2011, 499:208-211.

引证文献1

重庆医科大学学报
2011年 第4期

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