Twist、Snail、Slug在口腔鳞状细胞癌中的表达及其与各临床病理因素的关系

Expression of Twist,Snail,Slug and its significance in oral squamous cell carcinoma progression

目的：探讨Twist、Snail、Slug在口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）中的表达水平及其与各临床病理因素之间的关系。方法：采用免疫组织化学方法检测术前未接受放化疗的有颈部淋巴结转移或无颈部淋巴结转移的60例原发OSCC患者肿瘤切除石蜡标本中Twist、Snail、Slug蛋白的表达情况,检测结果采用SPSS10.0软件包进行统计学分析。结果：60例OSCC组织标本中,Twist的表达率为70%;Twist在颈部淋巴结转移的OSCC标本中的表达率为64.3%,无颈部淋巴结转移OSCC中的表达率为35.7%;早期OSCC中的表达率为55.6%,晚期OSCC的表达率为81.8%。Twist表达水平在OSCC患者有无颈部淋巴结转移和不同肿瘤分期之间具有显著性差异（P=0.012,P=0.046）。60例OSCC患者随访时间37个月（6~59个月）,16例患者术后复发。Twist表达与肿瘤复发相关（P=0.025）;Cox回归模式分析表明Twist蛋白表达是OSCC患者预后的独立预测因素之一（P=0.042）。与患者年龄、性别、T分期、病理分级、吸烟、饮酒等无关（P〉0.05）。60例OSCC组织标本中,Snail和Slug表达率分别为63.3%,58.3%;表达水平在OSCC不同临床病理因素之间的差异无统计学意义,对预后无影响（P〉0.05）。结论：Twist、Snail、Slug蛋白在OSCC中高表达,Twist表达水平在OSCC患者有无颈部淋巴结转移和不同肿瘤分期之间具有显著性差异,Snail、Slug表达水平与OSCC临床病理因素无相关性。

PURPOSE： To investigate the expression of Twist,Snail and Slug in oral squamous cell carcinoma（OSCC） patients as well as its clinical and pathologic significance.METHOD： Twist,Snail and Slug expressions were analyzed in 60 patients with OSCC by immunohistochemistry（IHC）.The correlation between the expression of Twist,Snail and Slug and patients＇ clinicopathologic characteristics was evaluated,including prognosis.The data was analyzed with SPSS10.0 software package.RESULTS： Twist expression was detected in 70% of 60 OSCC patients.Twist protein expression was detected in 35.7% cases of primary cancer tissues without lymph node metastasis,64.3% cases of primary cancer tissues with lymph node metastasis,55.6% cases of early stage primary cancer tissues and 81.8% cases of advanced stage primary cancer tissues.The presence of lymph node metastasis and tumor stage had association with Twist expression（P=0.012,P =0.046）.Snail and Slug expression were detected in 63.3%,58.3% of 60 OSCC patients.There was no significance correlation between Snail,Slug expressions and the clinicopathological parameters.During the follow-up period（6-59 months）,18 patients（30%,18/60） suffered tumor recurrence.Recurrences were found in 2 of 16 patients with Twist negative expression and in 16 of 42 patients Twist positive expression.A correlation between Twist protein expression and recurrence was detected（P=0.025）.In Cox regression analysis,Twist expression remained the only independent variate（P=0.042） and the other factors were not significant.But no significant correlations between Snail,Slug expressions and recurrence were found（P0.05）.CONCLUSIONS： Twist,Snail and Slug protein was overexpressed in OSCC.Twist expression was involved in OSCC progression.Twist may serve as a predictor for lymph node metastasis and poor prognosis.But,there was no significant difference between the expression of Snail,Slug and the clinicopathological parameters.