摘要
以纳米羟基磷灰石和壳聚糖为基质,构建一种新型甲硝唑缓释微球,作为充填材料用于骨修复。利用乙醇为反应溶剂,聚丙烯酸为分散剂,在pH=11的条件下,制备针状纳米羟基磷灰石。采用W/O型反相乳化一交联技术制备羟基磷灰石/壳聚糖载甲硝唑复合微球。通过紫外分光光度法测定甲硝唑含量和体外累积释放度。研究结果表明:制得的羟基磷灰石/壳聚糖载药复合微球粒径主要集中在1~10μm,壳聚糖对羟基磷灰石和甲硝唑形成了很好的包覆。复合微球平均载药量为38.23%,平均包封率为54.21%,3d内对甲硝唑的释放达到82%左右。所制备的羟基磷灰石/壳聚糖载药复合微球形态圆整,粒径分布较为均匀,对甲硝唑具有较好的缓释效果。
Nano-hydroxyapatite and chitosan were selected as matrix to prepare a novel metronidazole sustained release microsphere for bone tissue recovery. Aciform nano-hydroxyapatite was synthesized in ethanol medium (pH=11) using polyacrylic acid as dispersant. Metronidazole-loaded chitosan/hydroxyapatite composite microspheres were prepared by water in oil emulsion cross-linking method. The results show that the size of as-fabricated composite microspheres is mainly distributed from 1 to 10μm, and hydroxyapatite and metronidazole are embedded very well by chitosan. The average drug loading is 38.23%, the average entrapment efficiency is 54.21%, and the cumulative release rate to metronidazole is near 82% during 3 d. The composite microspheres are spherical and distributed uniformly in diameter with a good property for the slow-release of metronidazole, which have great potential in drug delivery system.
出处
《中南大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2011年第5期1232-1237,共6页
Journal of Central South University:Science and Technology
基金
国家自然科学基金资助项目(50872099)
关键词
缓释微球
甲硝唑
纳米羟基磷灰石
壳聚糖
sustained release microsphere
metronidazole
nano-hydroxyapatite
chitosan