肝细胞癌组织中HLA-DR,-DQ的表达及其意义

Expression of HLA-DR and HLA-DQ in Hepatocellular Carcinoma and its Significance

目的探讨人白细胞抗原(HLA)-DR和-DQ分子在肝细胞癌的表达及其意义。方法采用免疫组化法分别检测HLA-DR和HLA-DQ抗原在38例人肝癌组织芯片中的表达情况,并和临床病理资料进行相关性分析。结果肝癌组织中HLA-DR和HLA-DQ抗原信号主要定位于细胞浆。HLA-DR的阳性率为5.3%(2/38),HLA-DQ的阳性率为92.1%(35/38),但在4例伴淋巴结转移的肝癌中,3例无HLA-DQ的表达,HLA-DQ表达与肝癌的淋巴结转移负相关(P<0.0001)。HLA-DR和HLA-DQ表达与年龄和肝癌的组织分化无相关性(P>0.05)。结论 HLA-DQ的表达在肝癌组织中很常见,无HLA-DQ的表达可能提示与淋巴结转移有关。

To study the expression of HLA - DR and HLA - DQ antigens in hepatocellular carcinoma（HCC） and its significance. Methods The expression of HLA - DR and HLA - DQ antigens in human 38 HCCs cases were detected with immunohisto- chemistry, then the relations between the expression of genes with clinical and pathology parameters （ such as cancerous differentiation and metastasis and others） were analyzed by Chi square test. Results The immunoreactivity of HLA - DR and - DQ proteins were mainly dis- tributed in cytoplasm of cancerous cells, the positive rate of HLA - DR and DQ expression were 5.3% （2/35） and 92.1% （35/38） re- spectively. HLA - DR was related with gender of HCC patients, HLA - DQ was positive relation with non - lymphatic metastasis of HCC （P 〈 0. 0001 ）. Conclusion HLA - DQ expression is very common in HCCs; The absence of HLA - DQ expression may contribute to lymphnodes metastasis in HCC.