Bcl-2 和p53 蛋白表达对肝硬变和肝细胞癌中细胞增殖和凋亡的调节

Regulation of p53 and bcl 2 proteins to apoptosis and cell proliferation in liver cirrhosis and hepatocellular carcinoma

利用原位 Ｄ Ｎ Ａ 末端转移酶标记技术及 Ｓ Ｐ 免疫组织化学方法检测了肝硬变和肝细胞癌（ Ｈ Ｃ Ｃ）组织中凋亡细胞，ｐ５３ ， ｂｃｌ２ 蛋白和 Ｐ Ｃ Ｎ Ａ 的表达。结果显示： Ｈ Ｃ Ｃ 与肝硬变组织比较，凋亡细胞密度低， Ｐ Ｃ Ｎ Ａ 阳性细胞密度高，ｐ５３ 和 ｂｃｌ２ 蛋白阳性强度高，且均与 Ｈ Ｃ Ｃ 分化程度有关。提示ｐ５３ 和 ｂｃｌ２ 蛋白可能是通过其过度表达影响细胞增殖和细胞凋亡失衡，并以“选择性细胞增殖”形式导致 Ｈ Ｃ Ｃ 的发生和发展。

In situ apoptosis labelling was used for detecting apoptotic cells, and immunohistochemistry for p53, bcl 2 proteins and proliferation cell nuclear antigen(PCNA) in hepatocellular carcinoma(HCC) and liver cirrhosis tissues. The results were that in HCC, the number of apoptotic cells was higher, the density of proliferation cells lower, and expressions of p53 and bcl 2 protein were stronger than that in liver cirrhosis, and they were related to differentiation degree of HCC. The data indicate that overexpression of bcl 2 and mutant p53 proteins, which causes imbalance between cell proliferation and apoptosis, may bring about genesis and development of HCC by selecting proliferation of cells.$$$$