Hpa Ⅱ- P C R 检测慢性髓细胞白血病降钙素基因甲基化

Study of calcitonin gene methylation in chronic myeloid leukemia by using HpaⅡ PCR

采用 Ｈｐａ Ⅱ Ｐ Ｃ Ｒ 检测３１ 例不同病期慢性髓细胞白血病降钙素基因甲基化。结果显示：降钙素基因的高甲基化与慢性髓细胞白血病恶性程度密切相关，加速期与原始细胞危象期检出率远高于慢性期。提示检测降钙素基因甲基化水平对早期预测疾病转变和选择骨髓移植病例有参考价值。

To investigate the role of calcitonin(CT) gene hypermethylation in the transformation from the initial chronic phase to blast crisis of chronic myeloid leukemia, 31 CML patients were studied by using HpaⅡ PCR. The results showed that the 10.52%(2/19) chronic phase, 71.4%(5/7) accelerated phase and 80.0%(4/5) blast crisis of patients had CT gene hypermethylation and that the increased methylation of the CT gene were related with the disease progression. The reports indicated that the hypermethylation of CT gene might be a useful marker for predicting the evolution of CML and selecting chronic phase patients for BMT.