神经生长因子对大鼠脑缺血后海马CA_1区神经细胞凋亡的影响

Effect of nerve growth factor on neuronal apoptosis in hippocapal CA 1 sector after global ischemia in rats.

目的 研究神经生长因子（ Ｎ Ｇ Ｆ）在缺血性脑损伤中对神经细胞凋亡的影响。方法 采用大鼠 ４ 血管闭塞脑缺血模型， Ｔ Ｕ Ｎ Ｅ Ｌ 方法原位标记 Ｄ Ｎ Ａ 片段，观察脑室内注射 Ｎ Ｇ Ｆ 后海马 Ｃ Ａ１ 区神经细胞凋亡的变化。结果 使用 Ｎ Ｇ Ｆ 后 ３ｄ 海马 Ｃ Ａ１ 区 Ｔ Ｕ Ｎ Ｅ Ｌ 阳性神经细胞数为神经细胞总数的１３．９％ ±１１．６％ ，与缺血对照组（２１．３％ ±１３．７％ ）比较显著减少（ Ｐ＜ ０．０１）。结论 外源性 Ｎ Ｇ Ｆ 对脑缺血所致的神经细胞凋亡可能具有一定的保护作用。

Objective To investigate the protective action of nerve growth factor (NGF) on neuronal apoptosis by ischemic insults.Methods Global ischemia in Wistar rats was induced using four vessel occlusion,and NFG group intraventricular adminstration of NGF after ischemia,control group received the same volue of solvent.At the 3 days after ischmia,terminal deoxynucleotidyl transferase mediated dUTP biotion nick endlabeling (TUNEL) hippocampal CA 1 sector.Result The results showed that the number of TUNEL positive cells to the number of whole cells in the CA 1 subified was 21.3±13.7% in NGF group,and 13.9%±11.6% in control group,significant difference between the ratios in two group (P<0.01).Conclusion It was suggested that intraventricular NGF has a protective effect on neuronal appoptosis by ischemic insults.