白介素18基因型及血清水平与2型糖尿病肾病的相关性

The relationship of serum level and the genotype of interleukin-18 with diabetic nephropathy in type 2 diabetes mellitus

目的研究白介素18(IL-18)基因型及血清水平与2型糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测360例2型糖尿病患者(160例伴DN)及180名正常对照组的IL-18的基因多态性,同时采用酶联免疫吸附试验(ELISA)检测血清IL-18水平。结果与对照组比较,糖尿病肾病组血清IL-18水平显著高于对照组(P<0.01)。IL-18基因-137 G/C多态性在两组人群中的分布差异有统计学意义(P<0.05),携带 137 C等位基因的糖尿病肾病患者血清IL-18水平显著高于不携带者(P<0.05)。结论IL-18基因-137 G/C多态性与DN的发病可能具有相关性,其中C等位基因可能是DN发病的遗传易感基因;携带C等位基因的个体可能通过促进IL-18的高度表达进而增加了DN的发病风险。

Objective To study the relationship of the serum levels and genotype of IL-18 with diabetic nephropathy in T2DM. Methods The polymorphisms of IL-18 gene were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in 360 patients with type 2 diabetes mellitus and 180 healthy controls. And the serum level of IL-18 was determined by enzyme-linked immunosorbent assay （ELISA） . Results The diabetic nephropathy group showed significantly higher serum levels of IL-18 than control group （P〈0.01）. The IL-18 gene --137G/C polymorphism was significantly different between type 2 diabetic nephropathy and control group. The serum level of -137 C allele carriers was significantly higher than non-carriers （P〈0.05）. Conclusion IL-18 gene --137G/C polymorphism is associated with diabetic nephropathy in T2DM, and C allele may be a risk factor for diabetic nephropathy. The IL-18 C allele carriers may increase the risk for pathogenesis of type 2 diabetic nephropathy by enhancing the IL-18 expression.