不同营养条件对草地螟球孢白僵菌生长的影响

Effects of nutritional conditions on growth of Beauveria bassiana

研究了不同碳源、氮源和微量元素对3个草地螟(Loxostege sticticalis)球孢白僵菌(Beauveria bassiana)菌株菌丝生长及孢子产量的影响。结果表明,3种菌株均以有机碳作为碳源,且以葡萄糖为最佳碳源,蔗糖次之,而对于无机碳仅Bb-S05菌株可少量利用。氮源以酵母粉培养效果最佳。当C∶N=40∶8时,3个菌株菌丝生长和孢子产量最高。Fe3+、Mn2+微量元素组合对Bb-D01促进作用最大;Fe3+、Zn2+组合,Fe3+、Zn2+、Mn2+组合对Bb-S05有促进作用,而且Fe3+、Zn2+、Mn2+组合效果最佳;对Bb-Y03而言,添加任何单元素或多元素组合对其菌落生长均有抑制作用。

The effects of different carbon sources,nitrogen sources and trace elements on the growth and spore production of three kinds of Beauveria bassiana on meadow moth were studied.The results showed that organic carbon sources were suitable for three kinds of B.bassiana.The best organic carbon source was glucose and followed by sucrose.For inorganic carbon source,a few of which were taken only by Bb-S05.The best nitrogen source was yeast powder.The highest growth and spore production of three B.bassiana were obtained when C∶N=40∶8.For Bb-D01,the mixture of Fe、Mn addition showed the best facilitation.For Bb-S05,the most efficient mixture was the group of Fe、Zn、Mn and followed by the group of Fe、Zn.However,none of single trace elements or their mixture was useful to Bb-Y03.