人子宫内膜腺上皮和基质细胞的分离与培养

Isolation and cultivation of human endometrial glandular epithelial cells and stromal cells in vitro

目的:探索一种简便人子宫内膜腺上皮细胞和基质细胞的分离培养方法。方法:通过筛网法和差速离心法,分离培养5例子宫内膜腺上皮和基质细胞,利用免疫细胞化学染色及其激光共聚焦显微镜鉴定分离的细胞并进行图像分析。结果:用差速离心法分离子宫内膜腺上皮细胞中CK-19阳性表达积分为4.16±0.22,子宫内膜基质细胞中Vimentin阳性表达积分为4.24±0.22,与筛网法相比较差异有统计学意义(P<0.05)。结论:通过改良差速离心法使腺上皮和基质细胞分离步骤简化,是一种较理想子宫内膜细胞分离培养方法。

Objective To explore a convenient method of separating and culturing human endometrial glandular epithelial cells and stromal ceils in vitro. Methods Human endometrial giandular epithelial cells and stromal cells from 5 cases were separated by filtrated method and gradient centrifugation. The separate cells were identified by immunocytochemical staining and laser scanning confocal microscopy. Results The expression score of CK-19 in endometrial glandular epithelial cells was 4.16 ±0.22, and that of Vimentin in stromal cells was 4.24 ±0.22 in cells separated by gradient centrifugation, both were significantly different from those separated by filtrated method （P 〈 0.05）. Conclusion A simple and convenient method to separate and culture human endometrial glandular epithelial cells and stromal cells can be obtained by the modified gradient centrifugation. culture