利福平固体脂质纳米粒的体外细胞摄取研究

Preparation of Rifampicin-solid lipid nanoparticles and evaluation of their cell specificity

目的研究利福平固体脂质纳米粒的制备方法和体外细胞摄取。方法采用薄膜分散法制备利福平固体脂质纳米粒(RFP-SLNs);用扫描电镜观察其形态,激光粒度仪测定其粒径大小和Zeta电位;用MTT法测定其对RAW 264.7、A549细胞的毒性,HPLC法测定其在这两种细胞中的摄取量。结果 RFP-SLNs的外观形态圆整,为类球形,粒径为812.2 nm,Zeta电位为-38.4 mV;当RFP-SLNs的浓度不大于20.0μg·mL-1(RFP的含量)时,两种细胞存活率均大于80%。RAW 264.7、A549两种细胞对RFP-SLNs的摄取量分别为691.7、319.2 ng·mg-1。结论 RFP-SLNs能被RAW 264.7特异性摄取,具有体外的巨噬细胞靶向性。

OBJECTIVE To prepare Rifamipicin solid lipid nanoparticles and evaluate their cell specificity.METHODS Rifamipicin solid lipid nanoparticles（RFP-SLNs） were prepared by lipid film hydration method.The mophorlogy of RFP-SLNs was observed by scanning electron microscopy（SEM）.The diameter and surface charge of RFP-SLNs were measured by photo correlation spectroscopy（PCS）.MTT and HPLC were employed to evaluate the cytotoxicity and cell uptake of RFP-SLNs in RAW 264.7 and A549 cells,respectively.RESULTS RFP-SLNs presented sphere-like morphorlogy with diameter of 812.2 nm and Zeta potential of-38.4 mV.Cell viability of both RAW 264.7 and A549 cells was above 80% when concentration of RFP was not exceeding 20.0 μg·mL-1.The amount of RFP-SLNs absorbed in RAW 264.7 and A549 cells was 691.7,319.2 ng·mg-1,respectively.CONCLUSION RFP-SLNs showed higher uptake in RAW 264.7 cells,so it may be a good drug delivery system to macrophages.