拉曼光谱分析人类单个血小板的类胡萝卜素

Raman Spectroscopic Analysis of Carotenoids of Single Human Blood Platelets

应用拉曼光镊对人的单个血小板细胞(Plt)进行拉曼光谱收集与分析。拉曼信号显示,人类血液成分中,血小板的类胡萝卜素浓度最高。虽然类胡萝卜素在人类血小板中普遍存在,但是单个血小板之间差异极显著,且与血小板的脂类物质、蛋白质含量没有相关性。血小板激活过程引起蛋白质拉曼光谱的变化,而类胡萝卜素基本没有变化;常温(25℃)保存的血小板会导致类胡萝卜素光谱信号降低,脂类物质的相对拉曼信号强度基本没有变化,但经低温(4℃)12 d保存的血小板则相反。

The Raman spectra of single blood platelets from human body are acquired by using Raman tweezers and the biochemical information of Raman spectra is extracted. The carotenoids concentration of platelets is found to be highest among the blood. But the carotenoids concentration of platelets is heterogeneous and is irrelevant to the content of lipids and protein of platelets. After platelets is activated, the Raman intensities of carotenoid are constant but those of proteins arise. The Raman intensity of carotenoids decreases but the relative intensities of lipids remain stable when platelets are stored at 25 ℃. The Raman intensities of lipids stored at 4℃ 12 d decline while those of carotenoids unchange.