乳腺癌腋窝阴性淋巴结内微小转移的免疫组化检测和临床意义

Immunohistochemical detection of micrometastases in axillary lymphnodes of node-negative breast cancer patients and its clinical significance

目的 探讨应用免疫组化方法检测乳腺癌腋窝常规检测阴性淋巴结内微小转移灶。方法 应用单克隆抗细胞角蛋白（ Ａ Ｅ１／ Ａ Ｅ３） 和抗上皮膜（ Ｅ Ｍ Ａ） 抗体，采用 Ａ Ｂ Ｃ 免疫组化染色，观察淋巴结内阳性表达。结果 ６７ 例患者的阴性淋巴结４７３ 枚中，有１６ 例（２３ ．９ ％ ） 共３７ 枚淋巴结中发现微小转移灶，其阳性和阴性病人的癌复发，转移和预后差异性均有显著意义（ Ｐ＜ ０ ．０５） 。结论 免疫组化技术对乳腺癌腋窝阴性淋巴结的检测具有十分重要意义。

Objectives Detection of micrometastases in resected lymphnodes of node-negative breast cancer.Methods Monoclonal antibodies against cytokeratin (AE1/AE3) and anti EMA were as probs,using ABC immunohistochemistry technique to detect positive expression.Results Occult nodal metastases was observed in 16 of 67 cancer patients (23.9%),and in 37 of 473 lymph nodes examined.Follow up of breast cancer patients showed that the prognosis,local recourrence and distant metastases of patients with positive group were significant difference (P<0.05).Conclusions The data suggested that the immunohistochemistry technique is important to detect micrometastases in axillary lymphnodes of node-negative breast cancer patients