类胡萝卜素产生菌种的鉴定

Identification of a Carotenoid Producing Strain

于福建红酒酒糟中分离、筛选得到1株编号为B-5的产色素菌株。对该菌株所产色素进行定性分析,结果表明该色素为类胡萝卜素;对菌株进行常规形态和生理生化特性分析,结果表明该菌株为单细胞,呈卵圆形,芽殖;在固体培养基上,菌落呈深红色,菌落表面湿润、粘稠,边缘整齐,易被挑起;在液体培养基中,产生沉淀。无子囊孢子;无假菌丝形成。葡萄糖发酵试验为阴性,硝酸钾试验为阳性,耐高渗试验为阴性,产类淀粉化合物为阴性,37℃生长为阳性。利用26S rDNA D1/D2区域序列分析法对该菌株进行序列比对鉴定,结果表明,该酵母菌的序列与粘性红圆酵母(Rhodotorula mucilaginosa)模式菌株的序列同源性100%,结合该菌株常规形态和生理生化特性,鉴定该菌株为粘性红圆酵母(Rhodotorula mucilaginosa)。

A pigment producing strain B-5 was isolated from red wine lees in Fujian.The pigment was qualitative analyzed and indicated that it was carotenoid.Morphologic,physiological,and biochemical characteristics of the strain were analyzed,and revealed that the cell of the strain was single,oval and budding,dark red colony with humid and sticky surface,regular edge on solid medium and easily be picked out from,producing deposition in liquid medium,no ascospore and pseudohypha.The strain showed negative response to glucose ferment,potassium nitrate test positive,hypertonic test negative,amyloid production negative,and grew at 37 ℃.Using 26S rDNAD1/D2 domain sequence analysis and comparison identification indicated that the strain and Rhodotorula mucilaginosa model strain had 100% homology,combined with morphologic,physiological and biochemical characteristics,the strain was characterized as Rhodotorula mucilaginosa.