莫能菌素对蚯蚓(Eisenia fetida)活体DNA的损伤研究

Effect of Monensin Exposure on DNA Damage in Earthworm(Eisenia fetida)

通过单细胞凝胶电泳实验研究了不同浓度莫能菌素暴露对赤子爱胜蚓(Eisenia fetida)体腔细胞DNA的损伤,结果显示,50mg.kg-1莫能菌素处理组尾部DNA含量值最大、尾长值最大、Olive尾矩值最大,分别为34.539%、107.736μm和29.354;随着莫能菌素暴露剂量的增加,尾部DNA含量、Olive尾矩和尾长损伤频率增加;尾部DNA含量对莫能菌素暴露最为敏感,对照组和各处理组的尾部DNA含量之间均存在显著性差异(P<0.05);对照组与15、25、50 mg.kg-1处理组的Olive尾矩和尾长损伤频率之间均存在显著性差异(P<0.05);暴露浓度与尾部DNA含量、Olive尾矩和尾长具有良好的剂量-效应关系(P<0.05)。实验结果表明,蚯蚓体腔细胞DNA损伤可作为指示莫能菌素影响的生物标志物,彗星试验是检测莫能菌素暴露对赤子爱胜蚓活体基因损伤的有效手段。

The effects of monensin on DNA damage in earthworm coelomocytes（Eisenia fetida） were studied at different exposure concentration by comet assay,which was also known as the single cell gel electrophoresis（SCGE） assay.The results showed that monensin could induce DNA damage of earthworm coelomocytes.The maximum value of tail DNA percent,tail length and olive tail moment were respectively 34.539%,107.736 μm and 29.354 at the concentration of 50 mg·kg-1.There were significant differences between the control and treatments（15、25、50 mg·kg-1） in tail DNA percent（P0.05）.The significant dose-effect relationships were found among monensin concentration,tail DNA percent,OTM and tail length（P0.05）.DNA damage could be as biomarker to indicate the effect of monensin.The comet assay was a useful tool to analyze DNA damage of earthworm and apply in genotoxicit diagnose and biomonitoring of monensin in environment.