转双价(Bt＋CpTI)棉种植对土壤速效养分和酶活性的影响

Effects of Transgenic Bt + CpTI Cotton Planting on the Soil Available Nutrients and Enzyme Activities

本研究利用三室根箱对棉花根部土壤进行分区采集,以转双价棉SGK321及其亲本常规棉石远321为研究对象,对3个生长时期(播种后40、50 d和60 d)不同根区(S1、S2和S3)土壤速效养分(硝态氮、铵态氮和速效磷)含量及酶(脲酶、碱性磷酸酶和过氧化氢酶)活性变化进行了对比分析。研究结果表明,与常规棉相比,转双价棉的种植促进了S2根区土壤中磷素向有效态的转化,使S2和S3根区土壤硝态氮含量下降,而对各根区土壤脲酶、碱性磷酸酶和过氧化氢酶活性及土壤铵态氮无显著影响。主成分分析结果表明,土壤速效养分和酶活性变化主要受生长时期的影响,转双价棉种植对其影响是非常有限的。

In this study,three-room rhizobox method was used to collect soil samples from different zones of cotton root.This experiment compared the effects of transgenic Bt ＋ CpTI cotton SGK321 and conventional cotton SHIYUAN321 on soil available nutrients（NH＋4-N,NO-3-N and available P） and enzyme activities（urease,alkaline phosphatase and catalase） at different zones（S1,S2,S3） in three growing stages（40 d,50 d and 60 d）.The results showed that,compared to the conventional cotton,planting transgenic Bt＋CpTI cotton improved soil P transforming to the available form in the S2 zone,while the content of NO-3-N decreased in the S2 zone and S3 zone.No significant differences were observed in soil activities of urease,alkaline phosphatase,catalase and NH＋4-N among three root zones.Principal component analysis showed that the changes of soil available nutrients and enzyme activities were mainly affected by the growth stages,the impact of transgenic Bt ＋ CpTI cotton on soil available nutrients and enzyme activities was very limited.