摘要
目的探讨Avastin对角膜新生血管(NV)的抑制作用。方法日本大耳白兔20只,以角膜缝线法制作角膜新生血管模型,观察4周。在角膜缝线1周后,实验组以浓度为4mg/mL的Avastin溶液滴眼,对照组以注射用水滴眼,均为每日2次,共28d。记录角膜新生血管出现的时间、生长的长度和数量。结果两组角膜新生血管的出现时间分别为(3.43±0.53)d和(3.14±0.69)d(P〉0.05);在缝线后14d、21d、28d,实验组角膜新生血管的面积均明显小于对照组(P〈0.05)。且实验组角膜新生血管在用药后明显变细。两组角膜荧光素染色在观察期间均未发现明显着色。结论Avastin滴眼能够有效抑制角膜新生血管的生长,并能在一定程度上使已有的新生血管消退,且未发现明显的毒副作用。
Objective To evaluate the effect of Aavastin to inhibit experimental corneal neovascularization. Methods Twenty rabbits were sutured on the superior cornea. Animals were randomly divided into 2 groups. After 7 days, 10 eyes in the treatment group were given Aavastin drops(4 rog/mi) twice daily, whereas 10 eyes in the control group received placebo ( normal saline drops twice daily). The occurrence and developement of CNV was observed on the 3,7, 14, 21,28 days after suturing by slit-lamp microscope, and the area of CNV was calculated. Fluorescein staining was detected for the integrity of corneal epithelium. Results The occurrence of CNV in group 1 was ( 3.43 ± 0.53 ) d and group 2 ( 3.14 ±0.69 ) d. No significant difference in the occurrence time of CNV was found between group 1 and group 2. The CNV areas of group 1 were significantly smaller than that of group 2 at every time poin ( P 〈 0.05 ). The diameter of CNV was significantly smaller after Avastin treatment. Conclusion Avastin can inhibit and restrain corneal neovascularization at some degree without significant side effects.
出处
《中华眼外伤职业眼病杂志》
2011年第5期321-323,共3页
Chinese Journal of Ocular Trauma and Occupational Eye Disease