Avastin抑制兔角膜新生血管的实验研究

Inhibition of corneal neovascularization by Avastin in rabbits

目的探讨Avastin对角膜新生血管（NV）的抑制作用。方法日本大耳白兔20只，以角膜缝线法制作角膜新生血管模型，观察4周。在角膜缝线1周后，实验组以浓度为4mg／mL的Avastin溶液滴眼，对照组以注射用水滴眼，均为每日2次，共28d。记录角膜新生血管出现的时间、生长的长度和数量。结果两组角膜新生血管的出现时间分别为（3．43±0．53）d和（3．14±0．69）d（P〉0．05）；在缝线后14d、21d、28d，实验组角膜新生血管的面积均明显小于对照组（P〈0．05）。且实验组角膜新生血管在用药后明显变细。两组角膜荧光素染色在观察期间均未发现明显着色。结论Avastin滴眼能够有效抑制角膜新生血管的生长，并能在一定程度上使已有的新生血管消退，且未发现明显的毒副作用。

Objective To evaluate the effect of Aavastin to inhibit experimental corneal neovascularization. Methods Twenty rabbits were sutured on the superior cornea. Animals were randomly divided into 2 groups. After 7 days, 10 eyes in the treatment group were given Aavastin drops（4 rog/mi） twice daily, whereas 10 eyes in the control group received placebo （ normal saline drops twice daily）. The occurrence and developement of CNV was observed on the 3,7, 14, 21,28 days after suturing by slit-lamp microscope, and the area of CNV was calculated. Fluorescein staining was detected for the integrity of corneal epithelium. Results The occurrence of CNV in group 1 was （ 3.43 ± 0.53 ） d and group 2 （ 3.14 ±0.69 ） d. No significant difference in the occurrence time of CNV was found between group 1 and group 2. The CNV areas of group 1 were significantly smaller than that of group 2 at every time poin （ P 〈 0.05 ）. The diameter of CNV was significantly smaller after Avastin treatment. Conclusion Avastin can inhibit and restrain corneal neovascularization at some degree without significant side effects.